Adipose Tissue Lipoprotein Lipase and Hormone-Sensitive Lipase
نویسندگان
چکیده
منابع مشابه
Hormone-sensitive Lipase and Monoglyceride Lipase Activities in Adipose Tissue.
Several workers (l-3) have reported that lipase activity assayed in homogenates of adipose tissue is increased by exposure of the tissue to epinephrine or to adrenocorticotropic hormone prior to homogenization. In general, the magnitude of the increases observed has been small compared to the large effects of these hormones on glycerol format,ion by the intact tissae. In studies of whole homoge...
متن کاملPositional specificity of hormone-sensitive lipase from rat adipose tissue.
Hormone-sensitive lipase, purified from rat adipose tissue (Fredrikson, G., Strålfors, P., Nilsson, N. O., and Belfrage, P. (1981) J. Biol. Chem. 256, 6311-6320), has been incubated with tri-, di-, and monooleoyl[3H]glycerol, and the acylglycerol reaction products were isolated by thin layer chromatography on silicic acid, impregnated with boric acid. Trioleoylglycerol was hydrolyzed with the i...
متن کاملStudies on the hormone-sensitive lipase of adipose tissue.
Sucrose gradient centrifugation has been used to examine the triglyceride lipases present in extracts of rat epididymal adipose tissue. The aqueous infranatant recovered between the pellet and fat cake of tissue homogenates which had been centrifuged at 40,000 g was shown to contain two types of triglyceride lipase activity. One of these appears in the 15s region and has been identified as the ...
متن کاملRapid assay for hormone-sensitive lipase activity of adipose tissue.
A highly specific and rapid assay for hormone-sensitive lipase activity of rat adipose tissue is described. The method employs emulsified 2,3-di-O-oleyl-[9,10-(3)H(2)]oleoyl glycerol as a substrate; it is very sensitive and is suitable for serial sampling.
متن کاملLipoprotein lipase of chicken adipose tissue.
In recent publications (l-3), the clearing factor of postheparin plasma has been identified with a lipoprotein lipase obtained from rat, rabbit, and calf heart and rat and rabbit adipose tissue. This enzyme catalyzes the hydrolysis of the triglyceride moiety of chylomicrons and low density @-lipoproteins. The hydrolysis of simple triglyceride emulsions is also catalyzed by lipoprotein lipase bu...
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ژورنال
عنوان ژورنال: Arteriosclerosis, Thrombosis, and Vascular Biology
سال: 1997
ISSN: 1079-5642,1524-4636
DOI: 10.1161/01.atv.17.10.2287